Similarly, when exposing samples to those same conditions (protocol A’), at TP3 we can observe inconsistencies in pSN genetic profile in healthy and ALS samples, showing in the ALS cells higher genetic expression levels of NTRK3 and DICER; lower levels in ETV1 and PVALB, and similar levels in RUNX3 expression (Fig. 4E and Fig. S1, protocol A’). This evidence concerns the gene RUNX3 and amyotrophic lateral sclerosis.