When comparing ALS cells to healthy cells, we see that exposing cells to the same condition (protocol A’), at TP3 ALS samples show a significantly lower expression of certain motor-related genes as compared to their relative healthy ones: ETV1, motor system cells (PVALB +), myelin (MBP +), and motoneurons (MNX1 +) (Fig. 4C and Fig. S1, protocol A’). Here, PVALB is linked to amyotrophic lateral sclerosis.