The addition of ROCK inhibitor Y27632 on top of the increment of NT-3 on the medium (protocol D compared to protocol A’, Fig. 4A and Fig. S2), induced an opposite effect between healthy and ALS samples in PRPH, GFAP, MBP and MNX1, causing in all a decreased for healthy cells (p < 0.001, p < 0.05, p < 0.001 and p < 0.01 respectively) and an increase in ALS cells (p < 0.0001, p < 0.01, p < 0.001 and p < 0.001 respectively). Here, MNX1 is linked to amyotrophic lateral sclerosis.