The addition of CHIR99021 on the medium on top of the increment of NT-3 (protocol B compared to protocol A’, Fig. 4A and Fig. S2), induced in healthy cells a decrease in NTRK1 (p < 0.01) and MNX1 (p < 0.01), and a decrease in DICER (p < 0.05) in ALS samples. The gene discussed is DICER1; the disease is amyotrophic lateral sclerosis.