B3GAT1 and amyotrophic lateral sclerosis: The immunostaining and flow cytometry (FC) analysis performed onto floating spheroids of healthy and ALS cells undergoing SN differentiation through different protocols at TP2 showed that all samples assessed express HNK1 (an enzyme involved in cell metabolism and frequently used for the characterisation of neural crest stem cells35,46,51), both analysed through immunostaining and through FC, as seen in Fig. S4.