Furthermore, the wound-healing assays indicated that circPLIN2 knockdown markedly reduced the wound-healing speeds of ACHN and OS-RC-2 cells, and the wound-healing speeds of ACHN and OS-RC-2 cells were even slower when miR-199a-3p was added, while ZEB1 overexpression significantly rescued the inhibitory effects of circPLIN2 knockdown and the addition of miR-199a-3p on the migration of ccRCC cells (Fig. 7E, F). This evidence concerns the gene ZEB1 and nonpapillary renal cell carcinoma.