In addition, actinomycin D, an inhibitor of transcription, was applied to determine the half-life of circPLIN2 in ccRCC cells, and the content of circPLIN2 decreased slowly over time compared with the linear PLIN2 transcript in 786-O cells cultured in the presence of 2 μg/mL actinomycin D, suggesting that circPLIN2 was more stable or had a longer half-life than its linear counterpart PLIN2 (Fig. 1G). The gene discussed is PLIN2; the disease is nonpapillary renal cell carcinoma.