CCK-8 cell viability assays showed that circPLIN2 knockdown significantly inhibited the proliferation of ACHN and OS-RC-2 cells, while overexpression of c-Myc or MARCKSL1 significantly promoted the proliferation of ACHN and OS-RC-2 cells, suggesting that overexpression of c-Myc or MARCKSL1 rescued the inhibitory effect of circPLIN2 knockdown on the proliferation of ccRCC cells (Fig. 5A, B). The gene discussed is MARCKSL1; the disease is nonpapillary renal cell carcinoma.