Furthermore, the results of wound-healing assays showed that circPLIN2 knockdown substantially decreased the wound-healing speed of ACHN and OS-RC-2 cells, while overexpression of c-Myc or MARCKSL1 markedly accelerated the wound-healing speed of ACHN and OS-RC-2 cells, revealing that overexpression of c-Myc or MARCKSL1 rescued the inhibitory effect of circPLIN2 knockdown on the migration of ccRCC cells (Fig. 5E, F). This evidence concerns the gene MARCKSL1 and nonpapillary renal cell carcinoma.