Taken together, overexpression of c-Myc or MARCKSL1 rescued the inhibitory effect of circPLIN2 knockdown on the proliferation, migration, and invasion of ccRCC cells in vitro, suggesting that c-Myc and MARCKSL1 mediated the regulatory effects of circPLIN2 on ccRCC development and progression. Here, MARCKSL1 is linked to nonpapillary renal cell carcinoma.