Moreover, the IgM test has been reported to have low sensitivity (Hunsperger et al., 2009; Vickers et al., 2015) when using it as a single biomarker where the negative results obtained from IgM test cannot completely resolve the suspects of dengue infection (Vickers et al., 2015), therefore IgM test is more suitable to be used to detect recent infection but not as a marker for acute infection diagnosis (Blacksell, 2012). Here, CD40LG is linked to infection.