Further investigation indicated that Tp53, Pten or Rb1 signaling pathway plays different role during gliomagenesis in transgenic mice and zebrafish, and their cooperation could result in high-grade astrocytomas (grades III and IV) in astrocytes and neural precursors in transgenic mice and zebrafish, suggesting that they have different functions for the initiation and progression of gliomagenesis 9, 26. The gene discussed is RB1; the disease is astrocytoma (excluding glioblastoma).