CD274 and hepatocellular carcinoma: To clarify the reason why PD-L1 presented different expression pattern among HSF1 positive liver cancer, we observed firstly the expression pattern of HSF1 and PD-L1 in a variety of HCC cell lines including Huh7, PLC, HepG2, MHCC97H, Hep3B, SNU354, SNU449 and JHH1 and found that the baseline expression was low for both HSF1 and PD-L1 in Huh7 cells; in contrast, the other HCC cell lines with high HSF1 harbored high levels of PD-L1 (Supplementary Fig.2).