Of note, it was also interestingly found that both for HSF1 and for PD-L1, the positive related genes were obviously enriched in the common biological process-protein binding; meanwhile genes negatively related with HSF1 were dominantly enriched in the regulation of complement activation (Supplementary Fig.3), suggesting the involvement of a potential candidate which cannot only be responsible for upregulated chaperon activity, but also contribute to downregulated complement activation in the process of HSF1-induced PD-L1 expression in HCC. This evidence concerns the gene HSF1 and hepatocellular carcinoma.