It is recently recognized that JAK-STAT signaling is closely associated with immune evasion and resistance to immune checkpoint inhibitors in HCC30,31 as well as the function of STAT protein family depends on SH2 domain and play a crucial role in immune homeostasis.32 Herein, we firstly observed the activation of JAK-STAT signaling in the condition of HSF1 expression alteration in HCC cells. Here, HSF1 is linked to hepatocellular carcinoma.