To assess whether reduced eosinophils in SAT and changes of macrophages in EAT, SAT, and BAT from Nccfl/flUcp1Cre mice were due to NCC depletion in BAT or secondary to the increased body weight gain, glucose intolerance, and insulin resistance in these mice, we measured the eosinophil, total macrophage, M1 and M2 macrophage, total CD4+ and CD8+ T-cell, Th1, Th2, Th17, and Treg cell counts in EAT, SAT, and splenocytes from Nccfl/flUcp1Cre and Nccfl/fl mice under a LFD. This evidence concerns the gene CD4 and Glucose intolerance.