What’s more, we verified the expression of different gene sets represented various treatments (not performed in HNSCC-immunotherapy cohort due to the lack of transcript data of relative genes), such as immunotherapy, anti-EGFR therapy, anti-FGFR3 therapy and anti-PPARγ therapy, and found that TLSs was intimately correlated only with immunotherapy (Fig. 8G). The gene discussed is EGFR; the disease is head and neck squamous cell carcinoma.