The five AML cells lines were; t(4;11)-positive MV4-11 cell line encoding MLL-AF4 fusion proteins simultaneously with FLT3-ITD mutation, t(4;11)-positive RS4;11 cell line, SHI-1 cell line expressing MLL-AF6 fusion protein, THP-1 cell line with MLL-AF9 rearrangement and MOML13 characterized by FLT3-ITD mutations. The gene discussed is AFF1; the disease is acute myeloid leukemia.