Bladder cancer cell lines T24 and renal clear cell carcinoma cell line 786-O cells with high levels of PAQR4 mRNA and protein expression (Fig. 8D,E) were further selected to be transfected with siRNA to target PAQR4, and the knockdown efficiency was verified by qRT-PCR and western blot (Fig. 8F,G). This evidence concerns the gene PAQR4 and urinary bladder carcinoma.