Lentiviral vectors‐mediated silencing of DEPDC1 expression in HOS and MG‐63 osteosarcoma cells (western blotting and real‐time PCR, Figure 2A,B) resulted in diminished cell proliferation (MTT assay, Figure 2C), cell cycle arrest (EdU assay, Figure 2D), increased apoptotic cell ratio (Annexin V staining, Figure 2E), repressed cell colony formation (Figure 2F), retarded cell migration (scratch assay, Figure 2G), and cell invasion (Transwell assay, Figure 2H). This evidence concerns the gene ANXA5 and osteosarcoma.