Our data demonstrated that VEGFA expression was significantly downregulated following EZH2 inhibition, and VEGFA protein levels were restored in glioma cells after co‐transfection with siSPRY4‐IT1 and EZH2‐overexpression plasmid, suggesting that SPRY4‐IT1 regulated VEGFA expression through the mediation of EZH2 in glioma cells. This evidence concerns the gene SPRY4 and central nervous system cancer.