While NMII strongly replicated in Acod1−/− BMM, treatment with IFNγ starting 4 h after the infection, when extracellular NMII were washed away, inhibited bacterial replication in both WT and Acod1−/− BMM as shown by qPCR for C. burnetii GE (Fig 2E) and immunofluorescence staining (Fig EV1). This evidence concerns the gene IFNG and infection.