To test this, we incubated SPZs with purified IgG from a malaria hyperimmune serum pool in the presence of NHS (functional complement), HIS (non-functional complement), C4bp-depleted NHS (functional complement), C4bp-depleted-HIS (non-functional complement) or C4bp-depleted NHS supplemented with physiological level of C4bp for 10 minutes at 37°C. This evidence concerns the gene C4BPA and malaria.