Based on the tumor growth delay curves after RT (Figure 1A), the following five time phases were determined to assess the dynamic alterations in PD-1/PD-L1 expression level and relevant immune cell compositions in the tumor microenvironment and spleen: baseline (non-irradiated status prior to RT), early (initial status immediately after the initiation of RT), middle (time period of RT effect with tumor shrinkage), late (stable status with maintained RT effect), and progression (delayed tumor regrowth observed with limited RT effect) (Figure 1B). The gene discussed is PDCD1; the disease is neoplasm.