However, in the short-term 2D cell culture of BC cell lines with molecular types of ER/PR, -Her2/neu, and ER/PR−, Her2/neu+, IORT had no significant effect on the proliferative capacity of seroma; although, it showed the significant effects on invasion assay on 3-D Matrigel and migration test (12, 13). The gene discussed is ESR1; the disease is breast cancer.