With CRISPR/Cas9 methods, we knocked-out gal 4 in murine KPC tumor-derived cell lines using guide RNAs (sgRNA) directed against the gal 4 locus (Gal 4–KO, Supplementary Fig. S2A) and the inert R26 locus as control (R26 control). Here, LGALS4 is linked to neoplasm.