To confirm that these epithelial ductal organoids mirrored the gal 4 expression pattern observed in vivo, we performed Western blot analysis of organoid lines established from normal pancreatic tissue, tissue with PanIN lesions, and invasive carcinoma (Fig. 2C) and could detect a similar increasing gal 4 expression with severity of the lesion. The gene discussed is LGALS4; the disease is invasive carcinoma.