Image analysis of the organoid/immune cell cocultures showed a clear difference in organoid growth and survival between scramble-control and gal 4–KD cells, where cancer cells expressing gal 4 (scramble controls) formed larger organoids after 120 hours (Fig. 6C, top), whereas gal 4–KD cancer cells formed smaller and fewer organoids (Fig. 6C, bottom) when cocultured with immune cells. This evidence concerns the gene LGALS4 and cancer.