CHCHD2 and metabolic dysfunction-associated steatohepatitis: We further found that the protein level of CHCHD2 was greatly increased in livers of mice with NASH induced by methionine/choline-deficient plus 45% fat diet (MCD/HFD) feeding for 4 weeks, as evidenced by both immunohistochemical staining and Western blotting (Figure 2, A–C).