TIMP1 and Hepatic fibrosis: However, Sirius red staining revealed that liver fibrosis was decreased by CHCHD2 deletion (Figure 3, F and G), which was further evidenced by the lower expression of fibrotic markers, including desmin (Des), collagen type I α1 (Col1a1), collagen type III α1 (Col3a1), and tissue inhibitor of metalloproteinase 1 (Timp1) (P = 0.0836) (Figure 3H).