To confirm the pro-tumor role of HN1L in ESCC, we constructed ESCC cell lines with continuous overexpression or knockdown of HN1L. Firstly, we analyzed the protein level of HN1L in six ESCC cell lines and one immortalized esophageal epithelial cell line NE1 with western blotting. This evidence concerns the gene JPT2 and esophageal squamous cell carcinoma.