We performed double immunolabeling of tissues from glioma tumor centers using an anti-TSP2 antibody to label the TSP2 protein, an anti-GFAP antibody to label reactive astrocytes, an anti-NeuN antibody to label neurons, and an anti-Iba1 antibody to label microglia to explore the source of the TSP2 protein. This evidence concerns the gene THBS2 and glioma.