We then expanded these experiments to include knockdown of c-Myc in HCC cell lines with high MNX1-AS1 expression (HepG2 and PLC/PRF/5) and overexpresssion of c-Myc in low MNX1-AS1 expressing lines (Huh7 and HCCLM3). The gene discussed is MYC; the disease is hepatocellular carcinoma.