Interestingly, comparison of the expression of the hypoxia-associated molecule CA9 in each group demonstrated that the hypoxic microenvironment stimulated CA9 expression in ENO1-con pancreatic cancer cells but had no effect in the ENO1-knockdown group (Supplementary Figures S6A–S6F and S7A, S7B). This evidence concerns the gene ENO1 and familial pancreatic carcinoma.