The main findings are that daidzein (or E2): 1- significantly decreased kidney congestion, FENa+, FEK+, urine calcium, and systolic and diastolic blood pressure in UUO rats, 2- inhibited UUO-induced miR-33a and miR-27a overexpression, 3- ameliorated glomerulosclerosis and renal cell apoptosis, decreased the RIF index and RFI; and 4- inhibited Col3A1 mRNA and protein expression. Here, COL3A1 is linked to glomerulosclerosis.