Among these pathways, we selected PI3K/AKT and EGF signaling pathways as potential upstream regulators of CHD6 since PI3K/AKT was prominently presented in both IPA (Fig. 2a) and gene set enrichment analysis (GSEA) (Fig. 2b), and EGFR signaling is highly activated in CRC. This evidence concerns the gene EGF and colorectal carcinoma.