Among these pathways, we selected PI3K/AKT and EGF signaling pathways as potential upstream regulators of CHD6 since PI3K/AKT was prominently presented in both IPA (Fig. 2a) and gene set enrichment analysis (GSEA) (Fig. 2b), and EGFR signaling is highly activated in CRC. The gene discussed is EGFR; the disease is colorectal carcinoma.