To examine whether the effect of CHD6-TMEM65 axis on mitochondrial function contributes to the development of colitis-associated neoplasia in the AOM/DSS model, we examined mitochondrial protein expression with IHC staining on colon tumor sections obtained from AOM/DSS-treated Chd6fl/fl and Chd6-knockout mice. This evidence concerns the gene TMEM65 and colitis.