ZFAS1 and osteosarcoma: In addition, we detected the expression level of Lnc-ZFAS1 in osteosarcoma cell lines U2OS and KHOS, as well as the human osteoblast cell line hFOB1.19 and found the expression of Lnc-ZFAS1 was significantly higher in U2OS and KHOS cells than in hFOB1.19 cell (Fig. 1C).