ZFAS1 and osteosarcoma: Moreover, the up-regulation of MMP-9, Vimentin and N-cadherin and the downregulation of E-cadherin were observed in Lnc-ZFAS1 overexpressed U2OS cells, and the contrast results were induced by Lnc-ZFAS1 depletion, implying Lnc-ZFAS1’ promotion effect on EMT in osteosarcoma (Fig. 3D).