For example, etanercept increased the abundances of the Cyanobacteria and Nostocophycideae classes and the Nostocales order; it decreased the abundances of the Deltaproteobacteria class and the Clostridiaceae family.17 Patients who received methotrexate (MTX) showed a reduced abundance of Enterobacteriales and partial community restoration, compared with the typical dysbiotic community in patients with RA.13,17,18 Additionally, the bacterial community is affected by the presence of rheumatoid factor (RF) or anti-citrullinated protein antibody (ACPA), which are markers used to classify RA. The gene discussed is PRTN3; the disease is rheumatoid arthritis.