The necessity of actin assembly for both E. faecalis and L. rhamnosus survival, and the involvement of Wnt5A therein, was separately demonstrated by actin assembly inhibitor-mediated loss of bacterial CFU that was considerably recovered by the addition of rWnt5A to the inhibitor-treated E. faecalis and L. rhamnosus harboring RAW 264.7 cell cultures during a 3-h incubation period following infection. The gene discussed is WNT5A; the disease is infection.