PFKL and hepatocellular carcinoma: In contrast, we overexpressed PFKL protein by transiently transfecting PFKL plasmid into Sk-Hep-1 and HepG2 cells for 12 hours and found that protein expression of YTHDF3 was increased (Fig. 8b), indicating that YTHDF3 and PFKL have a positive loop regulation in HCC.