On the other hand, PFKL positively regulates the expression YTHDF3 at protein level, not as a kind of kinase, PFKL and YTHDF3 interact with each other by EFTUD2, and PFKL protein positively regulates YTHDF3 protein expression via inhibiting ubiquitination of YTHDF3 protein by EFTUD2, establishing a connection between pre-mRNA splicing and m6A modification in HCC (Fig. 9f). The gene discussed is EFTUD2; the disease is hepatocellular carcinoma.