What’s more, confocal assays of three group with Sk-Hep-1 cells incubated PFKL and EFTUD2 antibodies (Pearson’s coefficient = 0.91, Mander’s coefficient = 0.966), YTHDF3 and EFTUD2 antibodies (Pearson’s coefficient = 0.63, Mander’s coefficient = 0.977), or YTHDF3 and PFKL antibodies (Pearson’s coefficient = 0.74, Mander’s coefficient = 0.991) also indicated that there was co-location between YTHDF3, PFKL and EFTUD2 (Fig. 9a), and multi-label immunofluorescence staining of different HCC patients further demonstrated that PFKL interacted with YTHDF3 via EFTUD2 at the protein level (Fig. 9b). Here, EFTUD2 is linked to hepatocellular carcinoma.