To explore how YTHDF3 regulates glycolysis, we studied expression of mRNA and protein of rates-limited enzymes of glycolysis, including hexokinase 2 (HK2, catalyzing glucose to glucose 6-phosphate), phosphofructokinase-1 (PFK1, catalyzing fructose 6-phosphate to fructose 1,6-bisphosphate), pyruvate kinase (PKM, catalyzing phosphoenolpyruvate to pyruvate) and lactic dehydrogenase (LDH, catalyzing pyruvate to lactate) after YTHDF3 knockdown or overexpression in HCC cells. The gene discussed is HK2; the disease is hepatocellular carcinoma.