Our results described the signature of plasma sEVs from HCC and the upregulated pathway of complement cascades (C1QB, C1QC, C4BPA, and C4BPB) and the coagulation cascade (F13B, FGA, FGB, and FGG), which might be the major factors contributing to the classification of HCC. The gene discussed is F13B; the disease is hepatocellular carcinoma.