Meanwhile, we traced the intracellular events of PTK7-GEMs in bladder cancer cell lines 5637 and T24, PTK7-GEMs-Cy5 was first co-localized with lysosomes after co-incubated with bladder cancer cells in 2 h, then followed by lysosomal escape at 4 h (Additional file 1: Fig. S17A and B), and the onset of apoptosis could be observed after 8 h (Additional file 1: Fig. S17C and D). This evidence concerns the gene PTK7 and urinary bladder cancer.