To determine whether this is also the case in chronic fibrotic livers, Gls1 expression was analyzed: (i) in mouse and human primary HSCs and rat myofibroblastic HSC cultures, (ii) in mouse models of liver fibrosis (induced by methionine-choline deficient diet or CCl4 administration), (iii) in transcriptomic data derived from microarray analysis of over 70 NASH patients with varying degrees of liver fibrosis [138], and (iv) in liver biopsies from healthy human livers and patients with histologically graded fibrosis. The gene discussed is GLS; the disease is Hepatic fibrosis.