First, subcellular protein fractionations revealed that β-catenin expression in the nuclear component was significantly upregulated in the CEBPA-DT overexpression hepatoma cells and down-regulated in the CEBPA-DT silencing cells, while the expressions of β-catenin in the cytoplasmic fraction remained unchanged (Fig. 8A). The gene discussed is CEBPA; the disease is hepatocellular carcinoma.