To further validate that ProT upregulated HOTAIR expression through the NF-κB signaling, we silenced ProT expression in ProT-high-expressing TCCSUP bladder cancer cells via lentivirus-mediated delivery of shRNA specific to ProT, which was confirmed by RT-qPCR (Fig. 3e) and immunoblotting (Fig. 3f). This evidence concerns the gene SLC6A7 and urinary bladder carcinoma.