Quantitative real-time PCR (qRT-PCR) analyses of day 7 differentiated cells and FACS-enriched NCSCs further confirmed that the mRNA expression of CXADR and COL18A1 was indeed significantly upregulated in the DS group compared to that of the euploid NCSCs (Fig. 5a, b; Supplementary Fig. 10a, b). Here, COL18A1 is linked to Dravet syndrome.