The comprehensive systems biology validation of 9 hub genes has revealed that downregulated miRNA like hsa-miR-582-5p is correlated with the elevated the expression of ABHD2 and GSK3B genes in prostate cancer tissues, and upregulated miRNA like hsa-miR-548c-3p correlates with the lower expression of NR3C1 (glucocorticoid receptor) gene. The gene discussed is GSK3B; the disease is Familial prostate cancer.