Overall, SREBF1, LUC7L3, LAMA5, PCGF3, HNRNPH1, KLC4, and OFD1 were identified as possessing abnormal upregulation of alternative splicing, frameshift mutation, and NMD differential expression and were highly connected to both HNSCC prognosis and the infiltration of APCs (Figure 2G), which exhibit excellent properties of tumor antigens for the development of anti-HNSCC mRNA vaccines. The gene discussed is KLC4; the disease is head and neck squamous cell carcinoma.