We then detected the proliferation marker protein ki67 by immunohistochemistry (Liu et al., 2021), and the results showed that the expression of ki67 was significantly inhibited in the icaritin-treated group, which was consistent with the results of in vitro experiments, further demonstrating that icaritin can inhibit the proliferation of nasopharyngeal carcinoma (Figures 2E,F). This evidence concerns the gene MKI67 and nasopharyngeal carcinoma.