Consistently, the levels of Scr, BUN and the expressions of Kidney injury molecule-1 (Kim-1) and Neutrophil gelatinase-associated lipocalin (Ngal), AKI markers, were markedly elevated in AKI mice, which were significantly aggravated after EP4 inhibitor treatment (Figures 2D,E). The gene discussed is LCN2; the disease is acute kidney injury.