The results of Multi-label immunofluorescence assay showed that compared with the tissues of sensitive patients, the proportion of CD8+ T cells positive for MT2A, MT1E and MT1X was less in the esophageal cancer tissues of resistant patients, which were consistent with the results of single cell sequencing (Figure 5G, Supplementary Figures 1K, L). Here, CD8A is linked to esophageal cancer.