To evaluate changes in the architecture and function of peripheral immune system in one of the RIPK1-deficient patients, we performed mass cytometry (CyTOF) using a panel of 36 antibody markers, and compared the results to both maternal and paternal blood samples, non-IBD subjects (controls) and CD patients, An unbiased clustering algorithm (Phenograph) was performed on multiple immune populations. The gene discussed is RIPK1; the disease is inflammatory bowel disease.