In this study, CYT scores and TUBA1C were positively correlated in most cancer types, especially in LGG, OV, GBM, and BLCA (Supplementary Figure 5), which indicates that TUBA1C affects the prognosis of patients by affecting the activity of immune cells and the immune microenvironment. This evidence concerns the gene TUBA1C and bladder transitional cell carcinoma.