In this study, TUBA1C methylation was found to be associated with TUBA1C mRNA expression in a majority of tumours, and its hypermethylation resulted in better OS in PAAD, LGG, THCA, MESO, LIHC, and BRCA, which indicates the prognostic value of TUBA1C methylation. This evidence concerns the gene TUBA1C and pancreatic adenocarcinoma.