To evaluate the impact of METTL3-regulated DEPs on the prognosis of GC patients, firstly, LASSO regression and multivariate Cox analysis were performed on 173 DEPs to determine the best candidate DEPs, and 7 (DCK, TMEM164, ANXA5, OPLAH, NOS3, DNPEP, EPB41L3) independent prognostic DEPs were identified for building risk models, and the coefficients for each DEP in the risk model were -0.03, -0.02, 0.00, -0.01, 0.09, -0.01 and 0.04 respectively (Supplementary Table 3). The gene discussed is NOS3; the disease is gastric cancer.