In a three-dimensional organoid culture with M2-polarized macrophages mimicking the immunosuppressive tumor microenvironment, the cytotoxic capacity of mesothelin-targeted CAR-T cells was largely suppressed while mesothelin-targeted CAR-MAIT cells retained their potency against cancer cells, likely due to their direct recognition of tumor-associated macrophages (TAMs) through NK activating receptors and MAIT TCRs23 (Figures 1C and 2B). This evidence concerns the gene MSLN and cancer.