Gene profiling of single cells revealed that RGS5-expressing myofibroblasts, CXCL14-expressing fibroblasts and HHIP+ myofibroblasts showed extensive upregulation of extracellular matrix genes, including collagen genes, in colon cancer tissues (Fig. 4c, d, Supplementary Fig. 2a, b, Supplementary Data 5). This evidence concerns the gene HHIP and colonic neoplasm.