In the presence of autologous tumor cell membranes (TM), CD4+ and CD8+ T cells activated by FO or the crude lysates from FOLactis significantly had at least about a three-fold increase in surface expressions of CD69 and CD25 compared with the TM group (Supplementary Fig. 5d–g and Supplementary Fig. 6b–e). The gene discussed is CD69; the disease is neoplasm.