Compared with NCTD nano-micelle, anti-CA IX NCTD nano-micelle specifically binded to the highly expressed CA IX antigen at the tumor site, which can not only inhibit the catalytic activity of CA IX to prevent tumor deterioration, but also guide drug-loaded micelles to the tumor tissue, thus exhibiting enhanced antitumor effect on A549 cells and A549 tumor-bearing mice, with a tumor inhibition rate of 75.67% in vivo. The gene discussed is CA9; the disease is neoplasm.