In TGF-β1-treated ccRCC cells, lack of SKA1 increased the expression of the mesenchymal markers N-cadherin and vimentin while decreasing the amount of the epithelial marker E-cadherin, real-time PCR and western blot investigations (Figure 3B and 3C) reveal. This evidence concerns the gene CDH2 and nonpapillary renal cell carcinoma.