It turned out that in comparison to normal renal tubule epithelial cells HK-2, RCC cell lines 786-O, 769-P, ACHN, Caki-1, and OS-RC-2 displayed greater SKA1 mRNA and protein levels; nevertheless, endogenous SKA1 content did not significantly differ among these five renal cancer cell lines (Supplementary Figure 1A and 1B). Here, SKA1 is linked to renal cell carcinoma.