Although the DEGs in this study provide no direct evidence for HBPM increase, the upregulation of fbxl22 may indicate cardiac stress and vascular effect via upregulation of col22a1 and znfl2a, while col22a1 upregulation may be relevant to scoliosis phenotype at 4000 to 12,000 mg/L HFPO-DA exposures. Here, FBXL22 is linked to scoliosis.