Univariate analysis of risk factors that had statistically significant effects on the whole cohort with ALL were selected for inclusion in the multivariate analysis, and the results showed that WBC ≥50 × 109/L (EFS: HR = 2.7, 95%CI(1.1–7.1), P = 0.039; OS: HR = 4.4, 95%CI(1.1–19.6), P = 0.048) and MLL-AF4 (EFS: HR = 7.5, 95%CI (1.6–35.5), P = 0.012; OS: HR = 7.8, 95%CI(1.1–53.1), P = 0.036) were both independent risk factors for the prognosis of patients with ALL. The gene discussed is KMT2A; the disease is acute lymphoblastic leukemia.