CD8A and neoplasm: In a similar work, Cai et al. created an intelligent IL-12 nano-engineered CAR-T cell (INS-CAR-T) using a bioorthogonal reaction to deliver IL-12 nanoparticles to tumor tissues.226 The released IL-12 increased the secretion of anticancer cytokines and tumor chemokines, activated CD8+ CAR-T cells in tumors, and resulted in efficient antitumor immunotherapy.