To develop an in vitro model of PM disease, 3D-dECMs were repopulated with C1, C2, and C3 TDO, with mutational profiles resembling the most common gene alterations in CRC (C1 was KRAS-mutant, C2 was BRAF-mutant, while C3 had amplified FGFR1). The gene discussed is KRAS; the disease is colorectal carcinoma.